What's the difference between Knockout and Deletion experiments?.The TrueDesign tool provides links out to NCBI so you can easily view transcript alignments and can select the option that best meets your needs. You may want to choose to edit a unique region in a rare transcript, or you may want to target a conserved region across all transcripts. The answer to which transcript to choose will depend on the desired outcome of your experiment.How do I know which transcript to choose? I did a gene search and it displays multiple transcripts.To save your experimental details: you must export a results file, as your experiment will not be saved in the Cloud. ID3 EDITOR SAVE TEMPLATE FREETrueDesign is a completely free and online tool. ID3 EDITOR SAVE TEMPLATE VERIFICATIONIf you don’t immediately receive this verification request, check your spam folder. A verification request email will be sent to the email address on file for your online account. From your supported browser, log in to the Thermo Fisher Cloud using your existing username and password.The following browsers and their subsequent versions are supported: Internet 10 and newer, Google Chrome 23 and newer, Apple Safari 5 and newer, and Mozilla Firefox 16 and newer.Computer speed, internet bandwidth, and complexity of the design request will all factor into the speed of the design step. Depending on a multitude of factors, it may take anywhere from 30 seconds to >2 minutes for your design to load.How long do I have to wait after clicking “Design”?.Note that any de-selected products will not appear in the spreadsheet. You may also export your designs and results as an Excel spreadsheet. All other recommended products can be de-selected prior to adding the gene-specific items to your cart. The only products required to carry out the designed experiment are the CRISPR or TALEN reagents and the DNA Donor or TrueTag primers.Do I have to buy all the recommended products shown on the Summary page?. ID3 EDITOR SAVE TEMPLATE SOFTWAREThe TrueDesign software currently supports insertion and deletion workflows that can be managed with a 100nt ssDNA donor oligo. These experiments require a DNA donor template an excess of 100nt, usually double stranded DNA or a plasmid donor.Additionally, you can directly email a technical application scientist at What tools are available for insertion or deletion experiments with more than 30 base pairs? Click on this link to speak to a Thermo Fisher scientist and find out more about the TrueDesign tool.What do I do if I’m stuck or need additional help?.From this menu, click “account” and manage your preferences from there. If you already have an account, click on the person icon in the right-most corner to reveal a drop-down menu. When you create an account, there is an option to opt out of receiving any information about Thermo Fisher products and services via telephone, email, or any other electronic means.Will I get a lot of spam or promotional emails if I sign up for a Thermo Fisher account?.Utilize 1TB of free data storage, scientific analysis apps, and peer collaboration tools.Shop the online scientific Services Marketplace.Join the Aspire member program and receive a free, full-sized product.View and track existing or past orders and quickly reorder.Obtain account-specific pricing and online quotes.There are many benefits to having an account such as:.What else can I access with a Thermo Fisher cloud account?.If you have ever placed orders on the site before, you may already have an account. The only information you need to enter is first name, last name, email and a password.What information do I need to enter to make a Thermo Fisher account?.However, creating an account and/or signing in is completely free, easy, and quick. Since this software is maintained in the Thermo Fisher Cloud, there is a sign-in step.
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